Abstract: Aiming at the problems of component complexity of layer water, multi-type dosing, affecting the accuracy of chemical tracer test in Shengtuo Oilfield, three kinds of tracers' detecting accuracy about nitrate, thiocyanate, carbamide were analyzed. Combining with tracer detecting methods, the difference of test results among distilled water, injection water, produced fluid configured tracer was compared, and the method of making standard curve was proposed. The effect of nitrogen forming flooding foaming agent, injecting anti-corrosive agent, biocide, binary combination driving surfactant on tracer test results were researched. The result showed that carbamide tracer was less affected by various types of agents and its cost was lower, which was identified to be optimum tracer for Shengtuo Oilfield. The applicability of the method was verified by the field application.