Abstract: Sulfur is a widely used important chemical raw material, the quality of sulfur is determined according to the indicators and test methods stipulated in GB/T 2449.1-2014 Sulfur For Industrial Use-Part 1 : Solid Product and GB/T 2449.2-2015 Sulfur For Industrial Use-Part 2 : Liquid Product. In view of the limitations of the existing methods such as the content of iron and arsenic in industrial sulphur standards, new methods for the determination of arsenic content, iron content, hydrogen sulfide and hydrogen polysulfide content by spectrometry has been established. The results showed that: method for the determination of arsenic content in sulfur by atomic fluorescence spectroscopy has a detection limit of 0.005 μg/g, standard recovery rate of 94% to 103%, and comparison deviation with GB/T 2449.1-2014 less than 0.16 μg/g; method for the determination of iron content in sulfur by atomic absorption spectroscopy has a detection limit of 0.1 μg/g, standard recovery rate of 91% to 109%, and comparison deviation with GB/T 2449.1-2014 less than 1 μg/g; method for the determination of sulfur hydrogen and hydrogen polysulfide content in sulfur by infrared spectroscopy has a detection limit of 5 μg/g, measurement range of 5 μg/g to 500 μg/g and repeatability less than 8%. All the established methods meet the testing requirements. At the same time, the feasibility and applicability of methods for the determination of arsenic content, iron content, hydrogen sulfide and hydrogen polysulfide content by spectroscopy developed as national, industrial or enterprise standards are also discussed.